Entries by Bruno Zuercher

Harvesting plasmid DNA from high-density E. coli fermentation

With emerging fields such as cell and gene therapy and DNA vaccines, the demand for plasmid DNA is growing. Plasmids are commonly produced from E. coli and can be difficult to harvest, as the fermentations are often grown to extremely high cell densities. Depth filters foul easily and need to be replaced, and centrifuges struggle […]